PlasmidFactory GmbH & Co. KG products

Plasmid DNA in reproducible and certified quality for preclinical and veterinary research. Cultivation without use of animal derived media. Controlled removal of bacterial LPS endotoxins. Complete removal of RNA and proteins. A large number of quality tests already inclusive – without any additional charges.

In particular, the High Quality Grade production of plasmid DNA as a starting material for the production of RNA vaccines has gained in importance, especially in the context of the COVID-19 pandemic, as RNA is considered a promising vaccine for the prevention of certain virus infections and with the additional advantage of not integrating into the genome of the cell.

Plasmid DNA for preclinical applications in highly efficient ccc topology. Quality features like Research Grade and additional; Removal of the oc-plasmid forms: You receive the pure supercoil (>95%). CGE analysis inclusive (proof of the proportion of ccc forms)

Minicircles (MC) are circular DNA molecules that are generated by an intramolecular (cis-) recombination from a parental plasmid (PP). The difference between MC and standard plasmid vectors for gene therapy or nucleic acid vaccination is that the MC contains neither the bacterial origin of replication (needed only in bacteria for the amplification of plasmids in cell division) nor the antibiotic resistance markers or other selection systems.

High Quality Grade plasmid DNA can be produced according to the EMA guideline CHMP/BWP/2458/03 for the highest quality standards. For reasons of product safety the production process is done completely without animal derived substances, “animal-free”. High Quality Grade plasmid DNA guarantees the highest product purity through reliable separation of impurities, such as bacterial chromosomal DNA or damaged plasmids. To prevent further contamination, exclusively one plasmid is produced in our High Quality Grade plasmids manufacturing system, there are no parallel plasmid productions in one room at the same time.