SPINREACT products

Albumin in the presence of bromocresol green at a slightly acid pH, produces a colour change of the indicator from yellow-green to - green-blue. The intensity of the color formed is proportionalto the albumin concentration in the sample.

Bilirubin (both conjugated and unconjugated) couples with the diazo reagent in the presence of a surfactant to form azobilirubin. The intensity of color formed is proportional to the bilirubin concentration in the sample tested. The increase of absorbance at 546 nm is directly proportional to the total bilirubin concentration.

Bilirubin is converted to colored azobilirubin by diazotized sulfanilic acid and measured photometrically. Of the two fractions presents in serum, bilirubin-glucuronide and free bilirubin loosely bound to albumin, only the former reacts directly in aqueous solution (bilirubin direct), while free bilirubin requires solubilization with caffeine to react (bilirubin indirect). In the determination of indirect bilirubin the direct is also determined, the results correspond to total bilirubin. The intensity of the color formed is proportional to the bilirubin concentration in the sample.

The ASO-latex is a slide agglutination test for the qualitative and semiquantitative detection of anti-streptolysin O (ASO) in human serum. Latex particles coated with streptolysin O (SLO) are agglutinated when mixed with samples containing ASO.

The RF-latex is a slide agglutination test for the qualitative and semiquantitative detection of RF in human serum. Latex particles coated with human gammaglobulin are agglutinated when mixed with samples containing RF.

Anti-antithrombin III antibodies when mixed with samples containing antithrombinIII, form insoluble complexes. These complexes cause an absorbance change, dependent upon the antithrombin-III concentration of the patient sample, that can be quantified by comparison from a calibrator of know antithrombin-III concentration.

The Lp(a)-turbilatex is a quantitative turbidimetric test for the measurement of Lp(a) in human serum or plasma. Latex particles coated with antibodies anti-Lp(a) are agglutinated when mixed with samples containing Lp(a). The agglutination causes an absorbance change, dependent upon the Lp(a) contents of sample that can be quantified by comparison from a calibrator of known Lp(a) concentration.

FOB-Turbilatex is a quantitative turbidimetric test for the measurement of human hemoglobin in human feces. Latex particles coated with specific anti- human hemoglobin antibody are agglutinated when mixed with samples containing hemoglobin or hemoglobin/haptoglobin complexes. The agglutination causes an absorbance change, dependent upon the hemoglobin contents of the sample that can be quantified by comparison from a calibrator of known hemoglobin concentration.

Diluent is a buffer solution used to dilute blood and prevent cells agglutination. With correct osmotic pressure, ion pressure and the electric conductivity, it can maintain the integrality and original volume of the blood cells.

The assay is based on the reaction of creatinine with sodium picrate as described by Jaffé. Creatinine reacts with alkaline picrate forming a red complex. The time interval chosen for measurements avoids interferences from other serum constituents. The intensity of the color formed is proportional to the creatinine concentration in the sample.