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Based on UBA domains known to possess a high affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs have been demonstrated to protect ...
CISH (Cytokine Inducible SH2 Containing Protein) contains a SH2 domain and a SOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CISH may be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent ...
Protein Quantitation is Practical and Powerful Tracking or predicting maximal capacities for major photosynthetic and biosynthetic processes in mixed communities is possible with global antibodies and standards. Quantitative Immunoblotting Pairing your antibody with its quantified protein standard provides both a positive control and statistical rigor - ...