fusion-protein-tag Downloads
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Buffer BXT (10x) is a 10x concentrated Strep-Tactin XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin XT with very high affinity, but reversibly, and thereby allows the efficient elution of Strep-tag II or Twin-Strep-tag fusion proteins from the resin. Please note that Buffer BXT (10x) is not ...
Vectors for expression of fusion proteins possessing a (His)6-Tag are commonly used for simple purification of recombinant proteins by metal affinity chromatography (e.g. nickel-nitrilotriacetic acid). The MoBiTec His-Tag antibody is very useful in monitoring (His)6-Tag fusion protein expression and purification and shows a markedly increased sensitivity for (His)6-Tags compared with other ...
Expression vectors constructed to allow synthesis of fusion proteins between glutathione-S-transferase (GST) and proteins encoded by inserted foreign DNA fragments are widely used. Antibodies directed against fusion proteins possessing a GST-Tag are very useful for verifying recombinant protein expression as well as for immunoaffinity purification of such proteins expressed in E. coli. The ...