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ELISPOT assays are a key research tool for enumerating antigen-specific T and B cells in PBMC. As both T and B cells occur in major classes, immune monitoring has to be concerned with identifying these as well. So far, ELISPOT assays have been primarily done single or double color. We report the development of four color T and B cell ELISPOT assays. Cells secreting any of the four analytes can ...
SARS-CoV-2 neutralizing antibodies (nAbs) are special antibodies that function to prevent SARS CoV-2 from binding to the ACE2 receptor of human cells. High levels of nAbs indicate immunity against COVID-19. Standard IgG/IgM antibody tests – which are universally available – do not detect for nAbs and can only provide an indirect indication for immunity. A SARS-CoV-2 nAb test can be ...