western-blot-analysis Downloads
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Evaluation of the FastGene® Western ECL Kit as a chemiluminescent substrate for Western blot analysis compared to the manufacturer‘s competing ...
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Evaluation of FastGene® Block & Go as blocking reagent for western blot analysis of human protein expression in comparison to the standard protocol using 5 % non-fat dried milk as blocking ...
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FastGene® Block & Go is a blocking solution for Western blot analysis. This buffer not only provides blocking and primary and secondary antibody hybridization in a single-step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. Therefore, it serves as both blocker and enhancer in Western blot analysis. With the three-in-one ...
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Evaluation of the detection limit of FastGene® Western ECL Kit as chemiluminescence substrate for western blot analysis and detection of house-keeping genes from whole-cell lysates. In addition, detection sensitivity for primary antibody incubation for 1h at RT or overnight at 4°C was ...
In order to facilitate the operation and ensure the accuracy of WB results, the Western Blot Detection kit (Cat# E-IR-R304) is now available, containing the reagents which are needed from sample preparation to result detection. Please order the appropriate kit according to your specific ...
Western blot analysis of Cytokeratin 13 on human skin tissue lysate using anti-Cytokeratin 13 antibody at 1/1,000 dilution. ...
ByHuabio
Western blot analysis on different cell lysates using anti-LMNB2 rabbit polyclonal antibodies. Polyclonal antibodies (PAbs) are a mixture of antibodies that are secreted by different B cell lineages. These antibodies are actually a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope on an antigen. ...
ByHuabio
Western blot analysis of Cytokeratin 17 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. ...
ByHuabio
Western blot analysis of GARS on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. ...
ByHuabio
Western blot analysis of HIF-1 beta on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. ...
ByHuabio
Affinity Chromatography of His-tagged Protein on Qiagen Nickel Column2. Western Blotting on the Novex System3. Titration Curve on GE Healthcare “Phast” SystemThursday 7:30 a.m. - 5:30 p.m.¾ Demonstration: Preparative PAGE on BioRad Prep Cell¾ Morning Laboratory Exercises: Pour SDS Running Gel, Load Isoelectric Focusing Gel¾ Demonstration: “Phast” System, Native ...