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HuabioModel PRMT1 -JA11-17-ET1704-19 -Recombinant Rabbit Monoclonal Antibody

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ICC staining of PRMT1 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

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  • Product Type Recombinant Rabbit monoclonal primary
  • Product Name PRMT1 Recombinant Rabbit Monoclonal Antibody [JA11-17] (ET1704-19)
  • Immunogen Synthetic peptide within human prmt1 aa 12-61 / 371.
  • Host Rabbit
  • Positive Control D3, NIH/3T3, Hela, HepG2, SW480, human breast carcinoma tissue, mouse liver tissue, mouse colon tissue.
  • Conjugation Unconjugated
  • Clonality Monoclonal
  • Clone Number JA11-17
  • WB 1:500-1:1,000
  • ICC/IF 1:100-1:500
  • IHC-P 1:50-1:200
  • Form Liquid
  • Storage Condition Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
  • Storage Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Concentration 1ug/ul
  • Purification Protein A affinity purified.
  • Molecular Weight 42 kDa
  • Isotype IgG