Huabio
Huabio - Model PRMT1 -JA11-17-ET1704-19 -Recombinant Rabbit Monoclonal Antibody
FromHuabio
ICC staining of PRMT1 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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- Product Type Recombinant Rabbit monoclonal primary
- Product Name PRMT1 Recombinant Rabbit Monoclonal Antibody [JA11-17] (ET1704-19)
- Immunogen Synthetic peptide within human prmt1 aa 12-61 / 371.
- Host Rabbit
- Positive Control D3, NIH/3T3, Hela, HepG2, SW480, human breast carcinoma tissue, mouse liver tissue, mouse colon tissue.
- Conjugation Unconjugated
- Clonality Monoclonal
- Clone Number JA11-17
- WB 1:500-1:1,000
- ICC/IF 1:100-1:500
- IHC-P 1:50-1:200
- Form Liquid
- Storage Condition Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
- Storage Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- Concentration 1ug/ul
- Purification Protein A affinity purified.
- Molecular Weight 42 kDa
- Isotype IgG
