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Fetal Product Applications In Germany
5 applications found
Growth of FO myeloma cells in basal media containing SimPL-Serum. Growth of FO myeloma cells in media containing Simplified Platelet Lysate (SimPL-Serum) and fetal bovine serum (FBS). Cells were grown in standard DMEM basal media containing penicillin, streptomycin, and GlutaMAX™ with 10% (v/v) FBS or 10% SimPL-Serum in 6-well plates. Cells were grown in suspension culture and subcultivated every 3 days at an average cell density of 0.4 x 10^5 cells/mL. Data represent the total number of population doublings ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Growth performance of 3T3-L1 fibroblasts in media containing Simplified Platelet Lysate (SimPL) and fetal bovine serum (FBS). 3T3-L1 fibroblasts were grown in DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS or 10% SimPL. Media supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 5.7 mM Mg. 3T3-L1 cells were passaged using standard techniques in each condition 10 times before seeding a 96-well ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Growth performance of Madin-Darby canine kidney (MDCK NBL-2) cells in media containing Simplified Platelet Lysate (SimPL), fetal bovine serum (FBS), or human platelet lysate (hPL). Cells were grown in standard DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS, 2.5% SimPL, or 2.5% hPL. Media supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 2 mM Mg. Prior to growth evaluation, cells were ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Growth performance of human adipose-derived mesenchymal stem cells in media containing Simplified Platelet Lysate (SimPL) and fetal bovine serum (FBS). Cells were grown in standard DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS or 2% SimPL (left). Similarly, cells were grown in identical conditions except for the use of reduced-serum basal media (Gibco™ Advanced DMEM/F12) with either 2% FBS or 2% SimPL (right). Medias supplemented with SimPL also ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Platelets are one of the small components in blood circulation participating in primary hemostasis and thrombosis. Besides coagulation and wound healing, numerous research articles demonstrate the capability of human platelet lysate (hPL) in research, regenerative medicine and clinical applications. ...
ByAventaCell BioMedical Corp based in Atlanta, GEORGIA (US) (USA)