In Vitro Culture Articles & Analysis
16 articles found
These specialized cells play a crucial role in the filtration barrier of the kidneys, and their immortality allows for extended experimentation beyond the limitations of primary cell cultures. Podocytes are unique epithelial cells located in the glomerulus, a key component of the nephron responsible for filtering blood. ...
Various protocols exist for obtaining these cells from different sources, with bone marrow and adipose tissue being the most commonly used. Once isolated, hMSCs can be cultured in vitro under specific conditions that support their proliferation while maintaining their stemness. This ability to expand in culture is critical for producing ...
They exhibit high levels of keratinocyte markers, which are essential for maintaining the structural integrity and function of the corneal epithelium. These cells can be cultured in vitro, allowing researchers to study their behavior under controlled conditions. ...
The most important and critical step in cell culture is the selection of an appropriate growth medium for in vitro culture. ...
Using 2D cell culture systems can limit the predictive power of the cellular interactions. However, the 3D in vitro cultures provide a bridge between the conventional 2D cultures and in vivo studies. It was shown that in vitro 3D cultures recapture physiological cell-cell and cell-environment ...
These models help analyze molecular characteristics, control cell culture conditions, and facilitate functional testing. Moreover, they can further elucidate the targets and provide an effective environment for developing new drugs. The related systems of the services include but are not limited to in vitro 2D & 3D culture systems, ...
These models include in vivo animal models, in vitro cell culture models, in silico (computer-based) models, and genetically modified organism models. ...
Studies have shown that CPP-paclitaxel compounds are more effective than paclitaxel alone in both in vitro cell cultures and animal models. The activity of the drug was also observed in paclitaxel-resistant cells. ...
In fact, it is not difficult to see that these advanced technologies are all related to cell culture. In order to maintain efficient growth, proliferation, and differentiation during in vitro cell culture, many cell cultures require the addition of various cytokines. ...
Contexte Myasthenia gravis (MG) is an autoimmune disease caused by the presence of antibodies directed against components of the muscle membrane located at the neuromuscular junction. In the majority of cases, these are autoantibodies directed against the acetylcholine receptor (AChR). The origin of the autoimmune response is not known, but thymic abnormalities and defective regulation of the ...
Studies have also shown that CPP-paclitaxel compounds are more effective than paclitaxel alone in both in vitro cell cultures and animal models. In addition, drug activity was also observed in paclitaxel-resistant cells. 3. ...
The addition of BOC amino acids to the environment leads to the synthesis of the Cas9 protein, which stimulates the activity of the gene editing system. Using in vitro cell culture models and transgenic mouse models, the researchers demonstrated that this novel CRISPR/Cas9 gene editing system exhibits gene editing activity only in the presence of BOC unnatural ...
Developing an Inflammatory RepliGut® Model There are currently no in vitro models that completely model inflammatory diseases of the intestine. ...
Altis Biosystems tackled this challenge one step at a time. The first goal was to culture bacteria tolerant to oxygen on the RepliGut® system, which comprises mammalian cells grown on a two-dimensional surface separated by two accessible compartments — commonly known as a Transwell system, here cells are cultured on a permeable membrane with a ...
We preliminarily investigated the chemotherapeutic potential of pectin–cisplatin nanoconjugates, cisplatin and pectin on murine melanoma B16 cells in vitro and in–vivo. Mouse B16F10 melanoma cells were cultured in vitro in DMEM media containing 10% FBS, nonessential amino acids and antibiotics in a 5% CO2 incubator at 37°C and the effect of ...
This paper describes the irradiation response of lymphocytes, fibroblasts and tumour biopsies cultured in vitro, clonogenic survival at 2 Gy (SF2), chromosomal aberrations, and CD4 and CD8 lymphocyte apoptosis. ...