Rna Detection Articles & Analysis
11 articles found
RNA Modification Detection Detecting RNA modifications directly using ONT sequencing has also shown promise, although the resolution varies, and single-nucleotide resolution at the single-molecule level is yet to be demonstrated. In the past, PacBio sequencing was used to detect N6-methyladenosine (m6A) ...
Therefore, decoding information about modifications that affect sequence or structural changes is increasingly important. Methods for detecting RNA modifications Currently, most methods used to detect RNA modifications fall into three categories: high-throughput whole-transcriptome sequencing technologies, mass spectrometry ...
Traditional sequencing methods, and next-generation sequencing (NGS) technologies as well as emerging long-read sequencing platforms, have traditionally been employed for chimeric RNA detection. Traditional sequencing methods, like Sanger sequencing and reverse transcription PCR (RT-PCR), have been historically employed for chimeric RNA ...
Subsequently, a version of the PCR test called reverse transcription (RT)-PCR, detects RNA. This involves a two-step technique that typically consists of two enzymes; the first step utilizes a reverse transcriptase RNA-dependent DNA polymerase to copy RNA into DNA (cDNA), the second step then shifts to the use of Taq polymerase, ...
The RNA surveillance pathway detects and interprets defective transcripts to ensure RNA fidelity. ...
Small RNAs are important functional molecules in organisms, which have three main categories: microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA). They are less than 200 nt in length and are often not translated into proteins. Small RNA generally accomplishes RNA interference (RNAi) ...
Highlights Viable Delta Variant SARS-CoV-2 collected from air samples in a residential environment. SARS-CoV-2 detected in an area of a single-family dwelling distant from an isolation suite occupied by COVID-19 patient. ...
Cumulating data shows that RNA molecules, including mRNAs, lncRNAs, miRNAs, siRNAs, piRNAs, snoRNAs, and snRNAs, are more sensitive and specific than protein biomarkers since they are easy to detect and quantify at very low abundance. ...
Wastewater & COVID-19 Connections When the word wastewater crosses your mind, there is probably also a memory of your Mom’s voice telling you – wash your hands! When humans transitioned into community living, keeping your living space separate from your bathroom space was an important rule to learn. We can thank early scientists who were curious about how diseases spread and ...
One effort involves a method colorfully termed Mango, which has been developed on the back of a call from the Canadian Institutes of Health Research. Mango detects RNA molecules within living cells and comprises an RNA Mango aptamer that binds to a fluorescent dye. ...
The virus particles were recovered by glass wool filtration with elution using beef extract. DNA and RNA viruses were detected by the real time quantitative polymerase chain reaction (qPCR) and the reverse transcription-qPCR (RT-qPCR), respectively. HAdV-F and G were detected in 70.7% of the samples with concentrations up to 2.65 × ...