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Rna Sequencing Articles & Analysis
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Understanding sgRNAs sgRNAs are short RNA sequences that guide the Cas9 protein to the target DNA. Typically, they consist of approximately twenty nucleotides complementary to the target DNA sequence, paired with a scaffold region that recognizes and binds to Cas9. ...
Future Directions Advances in single-cell RNA sequencing and spatial transcriptomics are providing new insights into the tissue-specific expression of chemokines and their receptors. ...
This requires a comprehensive analysis of gene expression and the regulatory mechanisms at play during infection. Traditional RNA sequencing (RNA-seq) has long been employed to investigate the gene expression profiles of microbial pathogens. ...
Degradome sequencing, also known as parallel analysis of RNA ends (PARE) sequencing, is a cutting-edge technique that leverages high-throughput sequencing and bioinformatics to investigate the degradation products of mRNA. ...
A critical step in the NGS workflow is library preparation, which involves converting DNA or RNA samples into a form that can be sequenced. NGS Library Preparation Kits play a pivotal role in this process, ensuring high-quality libraries that yield optimal sequencing results. ...
These isolated cells were subjected to single-cell RNA sequencing to capture their individual transcriptomes. By combining surface marker profiles with indexes and transcriptomes, the authors now have a map of each cell type that can be used for later identification. ...
The next step is very similar to RNA-Seq. RNC sequencing is a method for profiling the Ribosome Nascent-chain Complex using next-generation sequencing (NGS) (RNC). ...
The backbone of clonality analysis is DNA and RNA sequencing, the most precise methods of determining the genetic makeup of cells. Through techniques such as next-generation sequencing (NGS), researchers can analyze huge volumes of genetic data, enabling the detection of even slight genetic variations among clones. ...
By cleaving RNA molecules, RNases are integral to numerous biological processes, ranging from RNA maturation to the innate immune response against RNA viruses. ...
Key techniques include multi-omics profiling, immune checkpoint blockers (ICBs), RNA-sequencing (RNA-Seq), single-cell analysis, imaging mass cytometry (IMC), and multiplexed ion beam imaging (MIBI). ...
We recently spoke with Manuela Badiali, Rita Congiu, and Stefania Murru from the Laboratory of Genetics and Genomics, Microcitemico Pediatric Hospital – A. Cao, Cagliari, about how SOPHiA DDM™ RNAtarget Technology has helped them optimize their variant analysis workflow. ...
This technique is based on two factors, guide RNA and Cas9 nuclease. Guide RNA alters its sequence for different target sites, while Cas9 functions as a nuclease cutting double strand DNA. ...
Dr. Kate Sasser from Tempus led a discussion on the potential of antibody-drug conjugates (ADCs), their challenges in targeting tumor antigens, and the future of cancer treatment with Dr. Daniel Johnson, Dr. Funda Meric-Bernstam, and Dr. Kellogg Parsons. The oncology field is rapidly advancing with the development of antibody-drug conjugates (ADCs), offering new hope for ...
ByTempus
What Is RNA Splicing? RNA splicing is a post-transcriptional modification process that occurs in eukaryotic cells. ...
Meanwhile, PNA monomers offer an arena of exploration beyond DNA and RNA. Peptide nucleic acids are synthetic polymers designed to mimic the behavior of DNA or RNA but are neutral and more chemically stable because they lack the negatively charged phosphate backbone. ...
In a recent Nature publication by Schappe,M (2024), researchers have revealed a fascinating aspect of respiratory physiology: the role of sensory neurons in guarding against airway obstruction and orchestrating crucial reflexes to maintain respiratory function. The study explores the intricate neural pathways involved in detecting and responding to airway closure. By characterizing a ...
This innovative technique promises to provide valuable insights into gene expression, protein synthesis, and regulation, offering potential advancements in the field of RNA translation research. Ribo-seq is a cutting-edge high-resolution sequencing technology that provides unprecedented insights into the mechanics of protein synthesis. ...
In this section, we explore the methodologies and tools developed for the detection of DNA and RNA modifications using ONT sequencing data. CD Genomics offers Epigenetics and Methylation Analysis Using Long-Read Sequencing for both DNA and RNA modifications. ...
Nanopore full-length transcriptome sequencing is an innovative technique that utilizes Oxford Nanopore Technologies' (ONT) sequencing platform to obtain comprehensive and uninterrupted high-quality full-length sequences of transcripts. ...
The backbone of clonality analysis is DNA and RNA sequencing, the most precise methods of determining the genetic makeup of cells. Through techniques such as next-generation sequencing (NGS), researchers can analyze huge volumes of genetic data, enabling the detection of even slight genetic variations among clones. ...