Single Nucleotide Polymorphism Articles & Analysis
16 articles found
How to Choose Sequencing read types: single-end versus paired-end reading? When it comes to sequencing DNA for genomics research, one critical decision that researchers must make is whether to use single-end or paired-end sequencing reads. ...
Variant detection and analysis refer to the sequencing and difference analysis of a genome from an individual or a population of a species using high-throughput sequencing technology in order to acquire a large proportion of genetic variation information, such as single nucleotide polymorphisms (SNPs), insertion and deletion (InDel), structural ...
Applications of Genome-wide Association Studies High-throughput genotyping technologies are used in GWA studies to test hundreds of thousands of single-nucleotide polymorphisms (SNPs) and link them to clinical conditions and traits that can be measured. ...
• Variant detection and allele-specific expression RNA-seq allows the identification of variants and allele-specific expression. Single-nucleotide polymorphisms (SNPs) refer to the variation in a single nucleotide that occurs at a specific position in the genome, which may lead to allele-specific expression ...
Variant call format (VCF) is the standard format for storing sequence variations, including SNPs (single nucleotide polymorphisms), indels, structural variants, and annotations. ...
It can discover new disease-causing variants and verify benign single- and multiple-nucleotide polymorphisms in human populations. Since 85% of disease-related mutations are found in the protein coding regions, WES is especially productive to study human diseases. ...
Finally, we used the spatial-ATAC-seq data to identify pixels with a single nucleus, which would be equivalent to scATAC-seq data (Fig. 3i,j). ...
Single-nucleotide polymorphisms (SNPs) are a kind of DNA polymorphisms caused by single-nucleotide variants in both coding and non-coding regions, which are the most common and smallest variants. ...
Its applications include variation and risk assessment in disease genomics, analysis of changes in transcriptomic gene expression, revealing epigenetic effects on tissue and organ development, and heterogeneity at the single-cell level, among others. We present the application of bioinformatics in the study and fight against cancer and infectious diseases. ...
Usually, targeted Illumina or 454 reads of up to a few hundred nucleotides are HTS methods to 16S rRNA sequence evaluation, each focusing on uniquely identifiable variable gene areas that can be utilized as unique microbial identifiers. ...
Genome-wide association study (GWAS) was conducted on a set of 199 wheat germplasm collected from Pakistan. In this study 31,000 single nucleotide polymorphism markers were developed by 90K SNP array technology. ...
Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. Multiplexing allows scientists to overcome limitations for myriad applications. First and foremost, multiplexing enables scientists to conserve valuable samples used in a quantitative assay, which is especially ...
According to Ruff, the key is to sort out antigen-specific single cells and determine their immunological profiles. Building on prior work (5), the team is now developing a functional tetramer—a construct comprising multiple molecules that can bind specific antigens—that binds to one of the dominant antigens in APS patients. ...
Single nucleotide polymorphisms (SNPs) are the most common sequence difference found in plant genomes, yet they have not been widely exploited for producing molecular markers in common bean (Phaseolus vulgaris L.). The objective of this study was to develop a SNP assay based on a type of heteroduplex mismatch cleavage called EcoTILLING for ...
A DNA microarray platform based on 2,200 genes from publicly available sequences was designed for Streptococcus thermophilus. We determined how single-nucleotide polymorphisms in the 65- to 75-mer oligonucleotide probe sequences affect the hybridization signals. ...
The analysis of specific DNA markers within the genome of an individual [single nucleotide polymorphism (SNP) genotyping] could potentially identify subjects at risk in clinical trials and may enable clinicians to individualise medical therapy. ...