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Sperm Articles & Analysis
11 articles found
The Cryopreservation Process The Cryopreservation Process is where biological matter (cells, tissue, organs, etc) is preserved by cooling to a very low temperature. This process stops the normal cell functions and holds the sample in suspended animation. When the time comes to thaw the sample, normal biological function resumes.Cryopreservation Process Steps The cryopreservation process can be ...
Chymotrypsin is a typical serine protease. As an endopeptidase that catalyzes the hydrolysis of protein peptide bonds, its realization form is mainly for the cleavage of the peptide bond formed by the carboxyl end of the aromatic amino acid-tryptophan, phenylalanine or tyrosine. Chymotrypsin is a typical serine protease and is currently one of the digestive enzymes of most vertebral animals. ...
There is a large and growing number of fertility clinics throughout the UK, all registered on the Human Fertilisation and Embryology Authority (HFEA) database. Each clinic is carefully monitored and assessed by the HFEA to ensure patients are protected and receive a service of high standard – even the clinic’s ‘Success Rates’ and ‘Inspection Reports’ are ...
Phthalic acid esters are frequently detected in aquatic environment. In this study, zebrafish were exposed to low concentrations (0, 0.46, 4.0, and 37.5 µg/L) of mono‐(2‐ethylhexyl) phthalate (MEHP) for 81 days, and the effects on reproduction, gamete quality, plasma vitellogenin (VTG), sex steroids, and transcriptional profiles of key genes involved in steroidogenesis were investigated. The ...
Bisphenol A acts as an endocrine disruptor, affects animal reproductive success in vivo, and affects sperm functions in vitro at environmentally relevant concentrations leading to reduction in sperm motility and fertilizing ability in fish. The effect of in vitro BPA on avian sperm functions has not been explored. We tested the effect of environmentally relevant concentrations of BPA (0, 0.18, ...
The effects of TBT on mortality and reproduction were studied in this invasive snail. The nominal fifty percent lethal concentration (LC50) was 9 µg TBT/L, after 28 days. The nominal ‘no observed effect on lethality concentration’ (NOECL) was 6 µg TBT/L after the same period. Male‐female couples and females that had been group‐mated but were isolated from males during the experiment (isolated ...
Attempts have been made to investigate the reproductive toxicity of norfloxacin drug in Japanese quail. Male quail were administered with norfloxacin @ 20 mg/kg body weight for 14 days. Then reproductive functions and androgen receptor (AR) gene expression was examined in treated and control birds. The results of the present study indicated that fertility, cloacal gland area, sperm ...
A tiered investigative approach was employed to characterize and identify substances responsible for the persistent toxicity of a municipal primary‐treated effluent to gametes of the Hawaiian sea urchin, Tripneustes gratilla. EPA Toxicity Identification Evaluation (TIE) procedures were modified to account for the tolerance of T.gratilla gametes to various sample manipulations. Microtox rapid ...
Adverse effects of bisphenol A (BPA) on male reproductive physiology were studied in male goldfish (Carassius auratus) exposed to nominal environmentally relevant concentrations (0.2 and 20 µg/L) for up to 90 d. Transcriptions of various reproductive genes were measured in brain, liver, and testis to investigate the BPA modes of action. Volume, density, total number, and motility and velocity of ...
Growing evidence shows that environmental estrogen can reach levels that are high enough to exert adverse reproductive effects on wild fish populations. The authors report different parameters of male reproductive behavior, brain, and gonadal aromatase expression, as well as sperm quality in an internally fertilizing fish species (Jenynsia multidentata, Jenyns) exposed to environmentally relevant ...
Freshwater turtles (Chrysemys picta) were collected from two sites on Cape Cod, MA. One site (Moody Pond), adjacent to the Massachusetts Military Reservation (MMR), was considered potentially impacted by toxic agents deriving from contaminant point sources on the MMR. The second (reference) site (Washburn Pond), to the east of the MMR, was considered not impacted by these pollutants and was ...