Western Blot Analysis Articles & Analysis
10 articles found
Therefore, detecting protein phosphorylation levels is of great significance for studying cell functions and disease mechanisms.Methods1. Western BlotThis uses phosphorylation-specific antibodies to detect the level of specific phosphorylation sites in protein samples.2. ...
Mass SpectrometryMass spectrometry provides a more comprehensive HCP analysis method, allowing researchers to identify and quantify individual HCPs, even at very low concentrations.3. ImmunoblottingSuch as western blotting, it's suitable for detecting specific, potentially high-risk HCPs.4. Risk AssessmentIn addition to quantitative ...
These forms of glycosylation affect protein folding, stability, and distribution.Methods and Techniques for Glycosylation Site DetectionTraditional glycosylation site detection methods include mass spectrometry analysis and Western blotting. Mass spectrometry can accurately identify glycosylation sites, while Western ...
Collagen is the most abundant protein in tissues and organs, and glycated collagen refers to collagen modified by glycosylation. Glycated collagen mainly exists in various tissues in the human body, especially those rich in collagen, such as skin, bones, joints, blood vessels, and other connective tissues.Under normal physiological conditions, glycation occurs as a natural biochemical process, ...
The overall workflow of Far-Western Blot technology is similar to Western Blot, but the detection of target proteins in Western Blot requires antibodies, while antibodies are not needed in Far-Western Blot analysis. Instead, purified and labeled ...
To detect protein ubiquitination, methods such as Immunoprecipitation (IP) combined with Western blot, mass spectrometry analysis, etc., are commonly used. Here are the typical steps for protein ubiquitination IP.Analysis Workflow1. ...
From protein immunoblotting to immunofluorescence, and then to FRET, antibodies are often an important determinant of successful and repeatable experiments. But we've never had a set "plan" to find the most suitable antibody. In this issue, the editor shares a 10-step "plan" for antibody selection.Determine the Name of the Target ProteinFirst, you need to be clear about what protein you need to ...
Immunoblot AnalysisPerform immunoblotting (Western Blot) analysis using antibodies against ubiquitin to detect whether Tau protein is ubiquitinated.5. Mass Spectrometry AnalysisFor accurate identification of ubiquitin sites on Tau protein, further mass spectrometry analysis can be performed.6. Data AnalysisAnalyze the ...
The GlueTACs constructed using this method were assessed for their degradation effect on target proteins using a non-small-cell lung cancer cell line, H460. Western blot analysis demonstrated a significantly higher rate of PD-L1 degradation induced by GlueTAC (remaining 11%) compared to NbTAC (remaining 68%), highlighting the crucial role of ...
The interaction between the two is confirmed by denaturing protein isolation and Western blot analysis. The GST pull-down experiment is an effective in vitro test technique to verify the yeast two-hybrid system. ...