Creative Bioarray products

Recommended Medium-RPMI Advanced medium supplemented with 2% fetal bovine serum.

SuperQuick® annexin V apoptosis detection kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate ofA nnexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.

The SuperQuick annexin V-EGFP apoptosis detection kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion ofAnnexin V, a protein that has a strong natural affinity for PS. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-EGFP and PI staining. Storage : 4°C.

The SuperQuick® annexin V-Biotin apoptosis detection kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, aprotein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using Any streptavidine- or Avidin-dye reagents.