SynBBB - 3D Blood Brain Barrier Model
SynBBB blood-brain barrier model recreates the in vivo microenvironment by replicating a histological slice of brain tissue cells. The model is able to do this by communicating with endothelial cells across the blood-brain barrier (BBB). As a result, shear-induced endothelial cell tight junctions are easily achieved in the SynBBB model using physiological fluid flow. This is something that cannot be achieved in other models such as the Transwell® model. The formation of tight junction changes can be measured using biochemical or electrical analysis (assessing changes in electrical resistance) with the SynVivo Cell Impedance Analyzer. Interactions between brain tissue cells and endothelial cells are readily visualized in the SynBBB assay. Transwell® models do not allow real-time visualization of these cellular interactions, which are critical for understanding the BBB microenvironment.
Highlights include:
- Accurate in vivo hemodynamic shear stress
- Real-time visualization and quantitation of cellular and barrier functionality
- Significant reduction in cost and time
- Robust and easy to use protocols
Chips: Depending on your specific research applications you can select from basic IMN2 (radial or linear) or IMN2 radial “TEER-compatible” chip configurations.
Kits: All the basic components required to run SynBBB assays can be purchased in a kit format. Two Kit formats are available, and you can choose between the IMN2 Radial, IMN2 Linear, or IMN2 Radial TEER Chips.
Starter Kit: Select this for your first-time purchase
- 10 SynBBB chips (Choice of IMN2 radial, IMN2 linear or IMN2 radial-TEER co-culture chips)
- Accessories including tubing, clamps, needles, and syringes
- Pneumatic priming device (required for priming tubing to remove air)
- Cell impedance analyzer* (required for SynBBB TEER measurements)
*included in the IMN2-TEER starter kit only
Assay Kit: Select this kit format if you have previously purchased the pneumatic priming device
- 10 SynBBB chips (Choice of IMN2 radial, IMN2 linear or IMN2 radial-TEER co-culture chips)
- Accessories including tubing, clamps, needles, and syringes