creative biogene - Gene Editing in Primary T Cells

CRISPR/Cas9 technology has been used as a genome-editing tool for multiple cell lines, such as tumor cell line and embryonic stem cell lines. Primary T cell, as an important class of immune cells system, play an extremely important role in immunity system. It holds great potential for therapeutic applications to engineer genome in primary T cells. However, genome engineering in primary T cells is still a challenge due to low delivery efficiency and limited expression of CRISPR/Cas9 components. In order to overcome these limitations, scientists have done many attempts and finally found an efficient method to overcome them. Nowadays, the widely adopted method is to transfect fully activated T cells with RNPs. Our scientists optimize this method in several details, which improves the efficiency.