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MtoZ Biolabs - Model systemic-lupus-erythematosus - Systemic Lupus Erythematosus Analysis Service
Systemic lupus erythematosus (SLE) is a classic systemic autoimmune disorder characterized by impaired autoimmune tolerance and high concentration of autoantibodies targeting self-antigens, which lead to organ damage. This pathological process involves multiple cytokines. Notably the role of CD40L (CD154) has been extensively studied in SLE, where CD40L is markedly upregulated. In both human and murine models, sustained CD40L expression in lupus T cells can drive excessive activation of B cells, contributing to the pathogenesis of disease. Elevated soluble CD30 levels in SLE serum mark for high disease activity. IL-10, a key Th2 cytokine, is implicated in this autoimmune dysregulation. Additionally, IL-13 has been a significant focus in SLE research. Increases in pro-inflammatory cytokines such as IL-6, IL-12, IL-18, and TNF-α can initiate the inflammatory process of SLE. These cytokines are found elevated expression in SLE patients and correlated with disease activity. It is well-documented that interferon-α levels are elevated in the serum of patients with active SLE, and this cytokine is responsible for the formation of vesicular inclusions in the renal endothelial cells of these patients.
Sarhan, D. et al. Int J Mol Sci. 2014.
MtoZ Biolabs employs flow cytometry for the simultaneous quantification of cytokines and proteins relevant to SLE, offering higher sensitivity and a broader detection range. The available cytokines for human samples are listed below and testing is also provided for other species. Free project evaluation!
1. Requires only 25 µL of Sample, Compared to 50 µL or 100 µL of Sample for ELISA Tests
2. Suitable for Use with Serum, Plasma, Cell Culture Supernatants, and Other Body Fluids
3. Samples of Body Fluids Should be Stored at -80°C and transported on Dry Ice
1. Experimental Procedures
2. Raw Data (Fluorescence Spectra)
3. Technical Report
4. Absolute Quantities of Each Assay Indicator (Excel Spreadsheets)
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com
Table 1. Systemic Lupus Erythematosus - Human Sample Multifactor Flow Cytometry