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Sample Preparation Articles & Analysis
38 articles found
Plant cell viability refers to the proportion of living, metabolically active cells in a given sample. It provides insight into cellular health, growth potential, and the effects of treatments such as cryopreservation, toxins, or genetic modification. ...
This application demonstrates the use of the R3/R5 microwave digestion systems to prepare samples of carbamazepine, an active pharmaceutical ingredient (API), for trace metal analysis. ...
Pharmaceutical manufacturers face growing challenges in ensuring product quality, maintaining regulatory compliance, and safeguarding against counterfeit drugs. Near-infrared (NIR) spectroscopy is an important tool in this arena, offering rapid, non-destructive analysis for raw material identification, impurity detection, and counterfeit screening. By integrating NIR spectroscopy into quality ...
The bioavailability and activity of glucosinolates in humans depend on factors such as food preparation methods, gut microbiota composition, and individual metabolic responses. ...
This method can accurately identify and quantify a large number of proteins in plasma samples. By analyzing protein expression patterns, changes, and interactions, researchers can gain valuable information on disease mechanisms, biomarkers, and therapeutic targets.A major challenge in plasma proteomics is the complexity of the samples and the dynamic range. ...
The study of gene TR changes in different samples is important for understanding the molecular mechanism of ncRNAs. ...
This technology identifies the diversity and specificity of T cells by measuring the sequences of the rearrangement regions of the TCRβ chain or α chain genes.(1) Sample Preparation: Total RNA or DNA is extracted from blood, tissue, or other samples enriched with immune cells.(2) Amplification: PCR amplification is performed using specific ...
Extract Antibody RNATotal RNA is extracted from the prepared monoclonal antibody samples.2. Reverse Transcription into cDNAThe RNA is transformed into cDNA using reverse transcriptase.3. ...
These techniques can separate, identify, and quantify collagen and its hydrolysis products in the samples.Common methods for collagen content determination include:1. Sample PreparationCollagen is extracted from the sample and preprocessed by centrifugation, filtration, or using Solid Phase Extraction (SPE) to remove impurities and purify the collagen.2. ...
Metabolomics Research Methods Metabolomics collects samples through established metabolomics research methods, and subsequently uses techniques to obtain metabolites, determine whether there are statistical differences in metabolites between groups and perform further analysis. ...
The medical industry is a new frontier for Raman spectroscopy. In the past few years, Raman has been used in dental and cancer studies, and is now building on this success by extension into Point-of-Care (POC) applications. This article gives an overview of some very new and exciting research into the use of Raman spectroscopy for the detection of cancerous tissue, biomarkers of disease, and ...
ByB&W Tek
This dynamic duo offers unparalleled insights into bile acid composition, concentration, and structural diversity. In LC-MS, bile acid samples are first separated based on their physicochemical properties, such as polarity and size, using liquid chromatography. ...
Supernatants were harvested and stored at −80°C for subsequent determination of virus titer and EC50 value. Cell lysates were prepared as described in the “Preparation of cell lysates and WES™ analysis” section for WES™ analysis. 2.4 Virus titer determination The viral titers of IAV and SARS-CoV-2 viruses were determined using ...
The cryogenic process and cryo storage are ideal solutions for the storage of biological samples and ensuring cell viability over time.Storing a sample under cryogenic conditions requires careful preparation to minimise damage to the sample. Considerations must be taken when deciding how to prepare a ...
Ion chromatography and sample filtration go hand-in-hand since this pretreatment step is advised for the majority of sample matrices. It is essential to avoid injecting particles, algae, or bacteria into the IC system, as these can jeopardize both the instrument and the separation column. Samples are often prepared manually, for ...
ByB&W Tek
Variant calling is used to identify SNPs and short indels in exome samples. The common variant calling tools are listed in Table 1. Some studies have evaluated these variant callers. ...
Introduction of HPLC-MS/MS-based Proteomics Technology The development of high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based proteomics technology mainly depends on the liquid chromatography-mass spectrometry equipment, sample separation and preparation technology, identification, as well as bioinformatics. ...
Since the 19th century, the practice of tissue processing has remained largely unchanged, resulting in solidified, paraffin-embedded tissue blocks for sectioning. As staining procedures have advanced and immunohistochemistry is becoming more widely used, high-quality tissue sections are paramount for accurate interpretation of disease ...
Quality control of hair, skin, and dentalcare products continues to increase in importance for producers. Aside from safeguarding our health, it is equally important to avoid the sale of out-of-specification products. Product recalls are very expensive and have a lasting effect on a company’s reputation. Therefore, intensive testing of these different care products is essential before ...
Finally, spatial-ATAC-seq is yet to be further extended to tissue samples from human patients with a disease to realize its full potential in clinical research. ...