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Dna Polymerase Articles & Analysis

7 articles found

qPCR for SARS-CoV-2 Detection and Research: Introduction, Principles, and Applications

qPCR for SARS-CoV-2 Detection and Research: Introduction, Principles, and Applications

Introduction to Quantitative Polymerase Chain Reaction A highly delicate technique for the amplification and identification of deoxyribonucleic acid is polymerase chain reaction (PCR). ...

ByCD Genomics


The Methods of Whole Genome Sequencing

The Methods of Whole Genome Sequencing

Two Classic Approaches for Sequencing Large Genomes In the early 80s, Sanger successfully completed a whole genome sequencing of the lambda phage by using the shotgun method, and the method was successfully applied to the larger virus DNA, the organelle DNA, and the sequencing of the bacterial genome DNA. ...

ByCD Genomics


Overview of Metatranscriptomic Sequencing: Principles, Workflow, and Applications

Overview of Metatranscriptomic Sequencing: Principles, Workflow, and Applications

And second strand of cDNA is synthesized by a DNA polymerase. Finally, sequencing adapters are attached to the cDNA strands, and this could be done either by PCR or by ligation. ...

ByCD Genomics


The Benefits of Lyophilized Reagents and Assay Kits (and Monkeypox Virus RT-PCR Kit)

The Benefits of Lyophilized Reagents and Assay Kits (and Monkeypox Virus RT-PCR Kit)

The first gene is the orthopoxvirus DNA polymerase gene (E9L) for the detection of Eurasian orthopoxviruses other than variola viruses, and the second one is a viral envelope protein gene (B6R), which specifically detects monkeypox virus (MPXV). ...

ByFireGene


Long Read Sequencing Fills in the Missing Pieces of Genomics

Long Read Sequencing Fills in the Missing Pieces of Genomics

The development of DNA sequencing technology has made it possible for human beings to explore the mysteries of themselves and other beings, while the advent of the genomics era has put higher demands on sequencing technologies. ...

ByCD Genomics


Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products

Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products

Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products Various microplates are available for quantitative real-time PCR in 96 well format. The main difference is the color, which significantly influences the real-time PCR signal quality and thus the results. A wide range of microplates from various manufacturers is available for real-time PCR in 96 well ...

ByAnalytik Jena - an EndressHauser Company


Removal of oxidative dna damage via fen1-dependent long-patch base excision repair in human cell mitochondria

Removal of oxidative dna damage via fen1-dependent long-patch base excision repair in human cell mitochondria

Repair of oxidative DNA damage in mitochondria was thought limited to short-patch base excision repair (SP-BER) replacing a single nucleotide. ...

ByAmerican Society for Microbiology (ASM)

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