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SARS-CoV-2 is a newly discovered human coronavirus group (HCoV), which are single-stranded RNA viruses belonging to the family of Coronaviridae. A range of respiratory diseases have been induced by some of these pathogens in the past. SARS-CoV-1 infected more than 8,000 individuals around the world, for example. Human coronavirus linked to Middle East respiratory syndrome (MERS-CoV) also occurred ...
Lyophilization enables developing countries to access reliable molecular biology reagents and kits without cold chain transportation. 2. Assay in one tube A pre-designed lyophilized PCR kit combines all individual components and assembles the precise amount of multiple PCR reagents for one reaction to a ...
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Mic qpcr cycler and Myra liquid handler have been put through their paces in COVID-19-testing field trials in regional Western Australia and found to be noticeably robust, rapidly deployable and flexible. These results have been outlined in a recent paper, Development, deployment and in-field demonstration of mobile corona virus SARS-CoV-2 Nucleic acid amplification test, published by Paton, ...
SARS-CoV-2 detection workflow in wastewater Monitoring wastewater for SARS-CoV-2 – or Coronavirus as it is more commonly known – can be a valuable data resource for an effective COVID-19 management. However, there are several challenges involved in detecting biological parameters in wastewater. Fortunately, there are also several solutions available to overcome them. Wastewater ...
Overview of the SARS-CoV-2 (Coronavirus) Detection Process In December 2019 the novel SARS-CoV-2 (formerly named 2019-nCoV) was identified in Wuhan, the capital of China’s province Hubei. Based on its rapid spreading with more than 105,000,000 confirmed cases by beginning of February 2021 the World Health Organization declared the outbreak a public health emergency of international ...
Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. Multiplexing allows scientists to overcome limitations for myriad applications. First and foremost, multiplexing enables scientists to conserve valuable samples used in a quantitative assay, which is especially ...
Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products Various microplates are available for quantitative real-time PCR in 96 well format. The main difference is the color, which significantly influences the real-time PCR signal quality and thus the results. A wide range of microplates from various manufacturers is available for real-time PCR in 96 well ...
Canadian fish‐based environmental effects monitoring programs use individual and population level endpoints to assess aquatic health. Impacts of coal mining and selenium (Se) exposure were assessed in slimy sculpin (Cottus cognatus) from reference streams located both inside and outside of a coal zone, and from one stream with a history of coal mining, using traditional environmental effects ...
In this study, we determined the removal of a prototype human norovirus (Norwalk virus, NV) by bench-scale alum coagulation, flocculation and sedimentation processes using reverse transcriptase polymerase chain reaction (RT-PCR) for norovirus assays. After determining optimum conditions for the coagulation, flocculation and sedimentation processes in terms of turbidity reduction, jar tests were ...
Yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. PCR-based methods have been used to detect Y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. In this study, quantitative PCR (qPCR)-based methods targeting the Yersinia virulence genes ail and yadA were used to survey the ...
The purpose of this study was to investigate the occurrence of antibiotic resistance genes (ARGs) in water used for irrigation in the Werribee River Basin, Australia, including river water and reclaimed effluent water (reclaimed water). Samples of reclaimed water, collected over a one-year period, were screened for the occurrence of ARGs using PCR detection assays. The presence of ARGs in the ...
The wheat (Triticum aestivum L.)-Aegilops geniculata introgression T5DL·5DS-5MgS(0.95), with stripe rust resistance gene Yr40 and leaf rust resistance gene Lr57, is an effective source of resistance against most isolates of the rust pathogen in Kansas and India. Rust resistance genes Lr57 and Yr40 were transferred to the hard red winter wheat (HRWW) cultivars Jagger and Overley by standard ...