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Ncardia - Ncyte Cardiomyocytes
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes are an in vitro platform that can provide a better solution for mechanistic analysis, and drug safety and efficacy screenings. Ncyte Cardiomyocytes are fully functional human iPSC-derived ventricular-like cardiomyocytes, obtained using Ncardia’s controlled bioreactor technology. Their relatively slow beat rate enables pacing for electrophysiological research. Ncyte Cardiomyocytes are a clinically relevant in vitro model compatible with an array of functional assays, such us calcium-imaging, contractility force evaluation and metabolism studies.
- High purity ventricular-like cardiomyocytes
- Physiologically relevant
- Highly suitable for electrophysiological research
Ncyte Cardiomyocytes are characterized by flow cytometry to ensure a purity of ≥ 70% cardiac TroponinT (cTnT) positive cells after 3 days in culture following Ncardia’s user guide (See figure A). They typically express Myosin Light Chain 2v (MLC2v), indicating a ventricularlike phenotype (See figure B). Based on RNA-seq data, Ncyte Cardiomyocytes express common sarcomere markers, such as TNNT2, while hiPSC and cardiac progenitor markers are silenced. Expression levels for additional genes are available upon request.
Ncyte Cardiomyocytes exhibit well aligned myofibrils and intact structural sarcomere organization (See figure A). Their action potential shows fast upstroke velocity and a well-defined plateau phase, resembling human ventricular cardiomyocytes (See figure B). These make the Ncyte Cardiomyocytes a physiologically relevant model for the phenotypic study of cardiac diseases. Our expert scientists can develop and execute predictive assays to support your drug discovery research in the cardiac field.
Ncyte Cardiomyocytes present a relatively slow and uniform beating rate, which enables pacing for electrophysiological research (See top figure). Additionally, response to adrenergic stimulation with isoproterenol and ion channel blockers, nifedipine (L-type calcium channel blocker) and dofetilide (hERG channel blocker), is guaranteed for every batch (See figure A, B, C). Our scientific team is experienced in the electrophysiological analysis of Ncyte Cardiomyocytes for safety and efficacy studies and can report the analyzed results to you.