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Hybridoma Sequencing Services
Absolute Antibody offers a quick and professional hybridoma sequencing service, also known as antibody sequencing. Hybridoma sequencing is the sequencing of the cDNA encoding the variable heavy (VH) and variable light (VL) domains of your hybridoma cell line.
Sequencing the antibody genes of your hybridoma offers many advantages:
- Security – your antibody can always be produced recombinantly even if your hybridoma line is lost
- Humanization – once you know the VH and VL sequences you can proceed to humanization if part of a therapeutic antibody project
- Flexibility – convert your antibody to any species, isotype or format
- Intellectual property – patent the sequences of your unique variable domains
5,000+ hybridomas sequenced.
At Absolute Antibody we perform high throughput (NGS) hybridoma sequencing. In 4-6 weeks, we deliver you all VH and VL sequences as well as a full report characterizing your hybridoma’s sequences and any heterogenicity or other antibody isotypes that might be present.
- Works on any species or isotype
- Use of random primers means that no mutations are introduced
- Identifies all HC and LC sequences and quantifies their relative abundance
- No sequence, no fee for any viable hybridoma
- Your sequence is your property – all our services are royalty-free
- High-throughput method can sequence up to 50 hybridomas concurrently – bulk hybridoma sequencing packages available
- Sequence delivered in 4-6 weeks
Even if a hybridoma is contaminated or unproductive, we could be able to save your antibody for recombinant expression. Review our collection of hybridoma sequencing case studies to learn how our unique NGS method can help overcome such obstacles to antibody production.