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Culture Medium Articles & Analysis

19 articles found

Analysis of Components of Antibody Drug Culture Medium

Analysis of Components of Antibody Drug Culture Medium

The main components of the culture medium include water, protein, sugar, amino acids, vitamins, minerals, and other growth factors. ...

ByMtoZ Biolabs


Analysis of Medium Components for Recombinant Protein Vaccines

Analysis of Medium Components for Recombinant Protein Vaccines

Since the production of recombinant protein vaccines involves the growth and expression of proteins in host cells (such as E. coli, yeast, mammalian cells or insect cells), the use of appropriate culture mediums is critical to successfully produce high-quality vaccines.Media component analysis is a crucial step in biotechnology and vaccine production. ...

ByMtoZ Biolabs


GMP Grade Cytokine Needed for Cell Therapy Manufacturing

GMP Grade Cytokine Needed for Cell Therapy Manufacturing

In order to make the cells grow well in the serum-free medium, a certain amount of appropriate cytokines, such as EPO, NGF, PDGF, EGF, M-CSF, etc., must be added. Serum-free medium needs to be free of virus contamination, animal-free, stable between batches, etc. ...

ByCreative Bioarray


The Six Main Ingredients in Cell Culture Medium

The Six Main Ingredients in Cell Culture Medium

The basic environmental requirements for optimal cell growth are: controlled temperature, substrate for cell attachment, appropriate growth medium and incubator to maintain correct pH and osmolarity. The most important and critical step in cell culture is the selection of an appropriate growth medium for in vitro culture. ...

ByCreative Bioarray


Pooled Human Cryopreserved Hepatocytes

Pooled Human Cryopreserved Hepatocytes

Hepatocytes are the chief functional cells of the liver, responsible for the selective uptake, metabolism and excretion of most drugs. Cells for use in plate cultures or suspension assays represent a key tool for predicting properties such as hepatotoxicity, metabolism, and potential drug-drug interactions. ...

ByCreative Bioarray


Influenza A virus replication has a stronger dependency on Raf/MEK/ERK signaling pathway activity than SARS-CoV-2

Influenza A virus replication has a stronger dependency on Raf/MEK/ERK signaling pathway activity than SARS-CoV-2

Twenty-four hours before the experiment, the growth medium was exchanged to medium with respective FBS or BSA concentration. ...

ByAtriva Therapeutics GmbH


What are Host Cell Proteins and Its Effect on Biopharmaceutical Development

What are Host Cell Proteins and Its Effect on Biopharmaceutical Development

Host cell proteases play critical roles in catalysis, metabolic pathways, and turnover of extracellular waste, but when present in the cell culture medium, proteases may lead to product breakdown. This phenomenon occurs in serum-free media, where proteolytic degradation occurs due to the lack of serum protease inhibitors in chemically defined media. During cell ...

ByCreative Proteomics


Construction and Culture Protocol of Neural Stem Cells Protocol

Construction and Culture Protocol of Neural Stem Cells Protocol

Transfer the culture medium containing nerve balls into a 15 mL conical tube. Clean the plate with a few of DPBS to maximize cell recovery. ...

ByCreative Bioarray


GMP Grade Cytokine Needed for Cell Therapy Manufacturing

GMP Grade Cytokine Needed for Cell Therapy Manufacturing

In order to make the cells grow well in the serum-free medium, a certain amount of appropriate cytokines, such as EPO, NGF, PDGF, EGF, M-CSF, etc., must be added. Serum-free medium needs to be free of virus contamination, animal-free, stable between batches, etc. ...

ByCreative Bioarray


Cultured Protein: A Guide to Every Cell-Based Meat Startup in the World Right Now

Cultured Protein: A Guide to Every Cell-Based Meat Startup in the World Right Now

In the group of startups focusing on enabling technologies the biggest subcategory is Cell Culture Media, where 2019 showed a real increase with a whole of eight new startups. This might be seen as the industry now being developed enough to show growing demand for cell culture media, and we’ve also heard multiple founders state that they started working on ...

ByAgulos Biotech, LLC


Module 3: Culture Medium

Module 3: Culture Medium

Learning objectives By the end of this module, you should understand: Basal culture medium composition and sources; Serum-free medium; The use of growth factors in culture medium. Basal culture medium composition and sources *Grammar/syntax note: The words for ...

ByAgulos Biotech, LLC


Vaginal lactobacilli inhibit growth and hyphae formation of Candida albicans

Vaginal lactobacilli inhibit growth and hyphae formation of Candida albicans

Lactobacillusstrains isolated from the vagina were cultured in MRS broth medium and subsequently filter-sterilised. The lactate concentration in the LCS was measured in accordance with the manufacturer’s instructions. ...

ByKoBioLabs


The creative potential of medium-sized and small Greek cities: reflections on contemporary cultural strategies

The creative potential of medium-sized and small Greek cities: reflections on contemporary cultural strategies

However, the cultural strategies of many medium sized and small cities focus more on social objectives. This seems to be the case for Greek cities of medium and small size. In order to determine the contemporary cultural strategies employed by local authorities and the State a survey was undertaken by the authors in 20 cities. ...

ByInderscience Publishers


Protective effect of curcumin on γ-radiation-induced sister chromatid exchanges in human blood lymphocytes

Protective effect of curcumin on γ-radiation-induced sister chromatid exchanges in human blood lymphocytes

Human lymphocytes were treated in vitro with 5.0 γg/ml of curcumin for 30 min at 37°C then exposed to 1, 2 and 4 Gy γ-radiation. The lymphocytes were cultured in (RPMI) 1640 tissue culture medium and autologous serum (20%). Phytohemagglutinin and 5-bromodeoxyuridine (10 pM) were added upon the initiation of culture and harvesting ...

ByInderscience Publishers


Entrepreneurship in Lebanon: a model for successes and failures

Entrepreneurship in Lebanon: a model for successes and failures

Entrepreneurs face different types of difficulties and operate in an unstable environment characterised by regional political chaos and local financial, legal, infrastructural, educational and cultural impediments. Small and Medium-sized Enterprises (SMEs) are the heart of any country; they empower its economy, heighten its competitive position, alleviate its ...

ByInderscience Publishers


Fibrinogen induces biofilm formation by streptococcus suis and enhances its antibiotic resistance

Fibrinogen induces biofilm formation by streptococcus suis and enhances its antibiotic resistance

In this study, we showed that supplementing the culture medium with fibrinogen induced biofilm formation by Streptococcus suis in a dose-dependent manner. ...

ByAmerican Society for Microbiology (ASM)


Chromosomal aberrations in radiation workers of radiology departments in Northern Iran-Babol

Chromosomal aberrations in radiation workers of radiology departments in Northern Iran-Babol

Blood samples were prepared from 62 radiology workers and 29 individuals as controls, and lymphocytes were cultured in a culture medium. Results showed that the mean frequencies of the total chromosomal aberration in radiation workers are significantly higher than those in controls. ...

ByInderscience Publishers


Development of an in vivo assay for detection of non-targeted radiation effects

Development of an in vivo assay for detection of non-targeted radiation effects

The serum from these samples was harvested, diluted in tissue culture medium and added to reporter cells. The toxicity or growth promoting activity of the serum was measured using a clonogenic assay coupled with immunocytochemical measurement of various proteins involved in apoptosis or growth. ...

ByInderscience Publishers


Effects on CD34+ haematopoietic stem cells induced by low doses of alpha irradiation

Effects on CD34+ haematopoietic stem cells induced by low doses of alpha irradiation

Radiation sources used are 222Rn and his daughters 218Po and 214Po previously dissolved in culture medium. CD34+ cells are exposed to alpha radiation and cultured for different times and doses, and later, survival, apoptosis fraction and total cell death (apoptosis and necrosis) are analysed. There are significant differences between irradiated ...

ByInderscience Publishers

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