Pcr Detection Articles & Analysis
13 articles found
Activation of the Raf/MEK/ERK signaling pathway has been shown to be an early and transient event in the replication cycle and was detected within the first 2 h post-infection in Calu-3 cells (Schreiber et al., 2022). ...
While RNA is like DNA, it is substantially different to imitate it very ineffectively with Taq polymerase, the conventional enzyme used for DNA amplification. Subsequently, a version of the PCR test called reverse transcription (RT)-PCR, detects RNA. This involves a two-step technique that typically consists of two enzymes; the first step ...
ELISA is a highly sensitive test that can detect small amounts of a specific protein or antibody in a patient's blood. ...
To this end, several diagnostic methods have been designed and tested, and one USA FDA approved test kit utilises fluorescence based reverse transcript real-time polymerase chain reaction (rRT-PCR) technique. The rRT-PCR test works by detecting replicated nucleic acid copies from the SARS-CoV-2 virus through a bound fluorescence probe that is ...
Lyophilized Monkeypox Virus Nucleic Acid Detection Kit In response to the 2022 monkeypox outbreak, we have developed our Lyophilized Monkeypox Virus Real-Time PCR Kit and obtained CE-IVD approval. ...
ByFireGene
The seroprevalence values for immunoglobulin type G antibodies against SARS-CoV-2 antigens obtained by rapid diagnostic testing on peripheral blood from volunteers reflect the current population-based estimates reported in the literature as well as the positivity rates detected by PCR-screening analyses. In conclusion, broad-based monitoring of IgG antibodies by ...
However, there are several challenges involved in detecting biological parameters in wastewater. Fortunately, there are also several solutions available to overcome them. ...
Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. Multiplexing allows scientists to overcome limitations for myriad applications. First and foremost, multiplexing enables scientists to conserve valuable samples used in a quantitative assay, which is especially ...
Plastic Makes the Difference: Ideal Real-time PCR Signals with Optimized Plastic Products Various microplates are available for quantitative real-time PCR in 96 well format. The main difference is the color, which significantly influences the real-time PCR signal quality and thus the results. A wide range of microplates from various manufacturers is available for real-time PCR in 96 well ...
Pathogenic Leptospira, the causative agents of leptospirosis, are mainly associated with tropical freshwaters, but little is known about their fate in the environment. This study analyzed the distribution of pathogenic Leptospira genomes in 22 tropical coastal streams using quantitative polymerase chain reaction (qPCR). Statistical analyses were conducted to understand pathogenic Leptospira ...
Monitoring of influenza viruses in sewage and surface water during the pandemic in 2009 showed that influenza A viruses were detected in sewage and surface water. However, the pandemic influenza A (H1N1) 2009 virus was not detected. ...
Samples of reclaimed water, collected over a one-year period, were screened for the occurrence of ARGs using PCR detection assays. The presence of ARGs in the reclaimed water samples were contrasted with that of water samples taken from the Werribee River Basin, collected over the same time period, from five points selected for varying levels of urban and ...
In this work, a preventive fast real-time PCR method for the detection, quantification, and identification of types of S. thermophilus phages in 30 min is ...