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ELISPOT assays are a key research tool for enumerating antigen-specific T and B cells in PBMC. As both T and B cells occur in major classes, immune monitoring has to be concerned with identifying these as well. So far, ELISPOT assays have been primarily done single or double color. We report the development of four color T and B cell ELISPOT assays. Cells secreting any of the four analytes can ...