Dna Target Articles & Analysis
19 articles found
This system allows researchers to target specific DNA sequences within a genome, facilitating the addition, removal, or alteration of genetic information. ...
Researchers design guide RNA (gRNA) that directs the Cas9 nuclease to a specific genomic location. Once the target gene is located, Cas9 induces a double-strand break, leading to disruptions in the gene’s function. ...
Conventional therapies often fall short in their ability to selectively target cancer cells without impacting healthy tissues. Alfa Cytology’s small molecule PARPi development services are specifically designed to overcome these limitations by delivering bespoke, high-quality PARP inhibitors tailored to the unique molecular characteristics of various cancers. ...
This process involves deleting, inserting, or modifying DNA in the bacterial genome, providing a powerful tool for studying gene function and genetic manipulation. The process uses nucleases that have been engineered to target specific DNA sequences, where they introduce double-strand breaks (DSBs) in the DNA molecule. ...
What is CRISPRi? The dCas9 fusion protein targets DNA sequences through sgRNA, but the inactive dCas9 cannot catalyze DNA. Instead, a protein fused to dCas9 manipulates the transcription of the target gene. When dCas9 is fused to the transcriptional repression domain Kruppel-associated box (KRAB), transcription is repressed, a ...
There are unlimited uses for point mutation cell lines at pre-defined loci, including functional assays, drug screening, FACS screening of membrane proteins, gene expression studies, gene therapy and antibody immunization boosting research. The RNA-guided DNA endonuclease Cas9 associates with a synthetic single guide RNA (gRNA) and cleaves double-stranded DNA ...
Protein fusion is a genetic recombination technique in which the DNA of an inactive peptide or protein chain segment to be grafted is recombined with the DNA of a drug and expressed together by engineered cells; no specialized grafting operations are required. ...
This process involves deleting, inserting, or modifying DNA in the bacterial genome, providing a powerful tool for studying gene function and genetic manipulation. The process uses nucleases that have been engineered to target specific DNA sequences, where they introduce double-strand breaks (DSBs) in the DNA molecule. ...
Recombinases are bacterial enzymes that can manage DNA sequences, yielding comprehensive, exact, and persistent gene control. Incorporating recombinase technology into AAVs develops unique rAAV particles that can target specific DNA sequences. Substantial strides have been made in constructing rAAV particles to transport the recombinase ...
Fluorescent Microspheres for Nucleic Acid Detection Microspheres encoded with up-converting luminescent materials of different colors can be used to bind different reporter labels, and when bound to single-stranded DNA, the fluorescence intensity will be different. A scientist used a similar method to wrap up-converting luminescent nanoparticles inside polystyrene microspheres, ...
Previous research has shown that the TnpB protein may act like a pair of molecular scissors, cutting DNA with the help of a special type of non-coding RNA called omega RNA. But how RNA-guided DNA cleavage works, and its evolutionary relationship to the Cas12 enzyme, was unclear, prompting research from Nureki's lab. ...
Facing the need for rapid detection of multiple pathogens at once, multiplex PCR followed by high-throughput sequencing is another method for routine targeted detection. This technique performs PCR reactions of multiple targets in one system by designing multiple pairs of PCR primers that specifically bind to the target region, thus achieving ...
Single-cell sequencing (SCS) can be used to obtain the sequence differences of cells in a specific microenvironment to facilitate the study of functional differences, etc. DNA sequencing of individual cells can help us understand small-scale cellular variation in cancer, for example, while RNA sequencing can help us understand and identify different cell types and the genes they ...
Next-generation sequencing (NGS) technology, which can sequence millions of DNA molecules simultaneously and in parallel, has rapidly attracted the attention of researchers over the past decade. ...
Advances in DNA sequencing technology have made it possible to extensively analyze the breast tumor genome and construct a catalog of gene mutations that may initiate or drive tumor progression. ...
Overview Confirm Cas9 mRNA expression With our ready-to-use Cas9 RT-PCR primer set, you can quickly and easily confirm Cas9 expression in cells or in vitro transcription assays. The amplicon for Game 1 is 219 bp and the amplicon for Game 2 2 is 122 bp. These primer sets allow the detection of nuclease Cas9, Nickase Cas9 and double mutant Cas9 at the messenger level. The primer sets are compatible ...
CHALLENGE The products for detecting human pathogens, utilize PCR technology, where target DNA segments are amplified, generating thousands to millions of copies of a particular DNA sequence. ...
The sensitivity of detection is increased by use of sensor array.Keywords: nanobiosensors, nanowires, nanosensors, biosensors, prostate cancer, cancer detection, sensor models, MATLAB, Nanohub, prostate-specific antigen, PSA, DNA, target molecules, sensor arrays, ...
Silencing of gene expression by siRNA holds a promise both for a drug target discovery and as a therapy. Major bottlenecks of siRNA technology include 'off-target' silencing effects and problems with siRNA degradation before delivery. ...